Gab1和Sfmbt2基因在成年普通牛8种不同组织中的印记状态分析

doi:10.11843/j.issn.0366-6964.2017.06.004

Abstract

Abstract:

In order to reveal the imprinting status of Gab1 (Grb2-associated binding protein 1) and Sfmbt2 (Scm-like with four mbt domains 2) in different tissues and placenta of bovine, in this study, we analyzed the imprinting status of Gab1 and Sfmbt2 in 7 bovine tissues (heart, liver, spleen, lung, kidney, muscle and fat) and placenta by direct sequencing the RT-PCR products based on single nucleotide polymorphisms.The result displayed that Gab1 and Sfmbt2 were all expressed in 7 tissues and placenta.We discovered that Gab1 and Sfmbt2 were bi-allelic expressed by comparing sequence chromatograms of RT-PCR products and genomic PCR products at single nucleotide polymorphisms sites, suggesting that Gab1 and Sfmbt2 were not imprinted in adult cattle tissues and placenta.

CLC Number:

S823
S813.3

WANG Guan-nan, ZHAO Yu-peng, CHEN Wei-na, ZHANG Cui, XU Da, LI Dong-jie, LI Shi-jie. Genomic Imprinting Status of Gab1 and Sfmbt2 in Different Tissues of Adult Cattle[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(6): 1000-1006.

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Genomic Imprinting Status of Gab1 and Sfmbt2 in Different Tissues of Adult Cattle

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